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Research & Reviews in Biotechnology and Biosciences

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TABLE OF CONTENTS

Paper Title:
Evaluation of Improved Mulberry Genotypes at Jammu in Rainfed Condition
Author Name:
Rajat Mohan, Anil Dhar, Dhiraj Vyas, Hilal Ahmad Bhat, Taran Deep Kaur
Country:
India
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Evaluation of Improved Mulberry Genotypes at Jammu in Rainfed Condition
Author: Rajat Mohan, Anil Dhar, Dhiraj Vyas, Hilal Ahmad Bhat, Taran Deep Kaur

Sericulture is an agro cottage industry wherein poor farmers are involved in producing cocoons through silkworm rearing by feeding mulberry leaves. This provides them additional income by working merely for about 25 days each in spring and autumn.100-200 disease free layings(dfls) , if reared in a crop will lead to production of 50-100 Kg cocoons @ of 50Kg /100 dfls which will fetch an income of 5-10000 in one crop (depending upon the dfls one rears).This requires qualitative , nutritious , palatable mulberry leaves. The local mulberry varieties grown by farmers do not yield leaves of such parameters which may suite to feeding silkworms. Therefore screening of suitable mulberry varieties is an immediate need. Scientists of Central Silk Board have developed hundreds of varieties but one variety performing better at a place may not perform better at other at other place and hence evaluation of such varieties is required at the specific place where it is to be grown.

Paper Title:
Microbiological Preparation of Non-Alcoholic Self Carbonated Beverage from Carrot and Its Blends with Lemon
Author Name:
Inderjeet Kaur, Heena Mandal
Author Email:
kaurinder@lkc.ac.in
Country:
India
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Microbiological Preparation of Non-Alcoholic Self Carbonated Beverage from Carrot and Its Blends with Lemon
Author: Inderjeet Kaur, Heena Mandal

A technology for preparation of non-alcoholic self-carbonated beverage from carrot and its blends with lemon juice using “Saccharomyces cerevisiae G” was done under optimized fermentation conditions. This yeast on inoculation @ 0.5% v/v in blended juice whose Total Soluble Solids (TSS) adjusted to 16.0°B and fermentation at 20 ± 5°C for 36 h produces a non-alcoholic naturally carbonated beverage. Two combinations of 1:1 and 3:1 of carrot and its blends with lemon juice were prepared. The physicochemical changes for carrot and lemon (1:1) beverage recorded after storage for three months at refrigerated condition (4⁰C) showed TSS around 14°B from 16°B, pH declined from 4.8 to 3.5, % titrable acidity increased from 1.69 to 1.85, Brix acid ratio reduced from 9.43 to 7.56, total sugars (%) decreased from 35.8 to 24.3 and reducing sugars (%) varies from 6.7 to 2.0. For carrot and lemon (3:1) beverage the physicochemical characteristics were TSS 13.5°B, pH reduced from 6.0 to 4.5, % titrable acidity increased from 1.83 to 2.24, Brix acid ratio declined from 7.95 to 6.37, total sugars (%) varied from 3.44 to 2.89 and reducing sugars decreased from 0.43 to 0.10 with a shelf life of 90 days under refrigerated conditions (⁰C). Sensory evaluation was also done after addition of spices like salt, mint and cumin in the ratio of 1:2:1. The carrot: lemon (1:1) beverage score „liked very much‟ and carrot: lemon (3:1) beverage score „liked moderately‟ due to naturally produced CO2 during fermentation that adds effervescence, sparkle and tangy taste to the beverage.

Paper Title:
Molecular docking and in silico analysis of cancer using Survivin as drug target
Author Name:
Gobind Ram, Ramandeep Kaur
Author Email:
ramgobind4@gmail.com
Country:
India
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Molecular docking and in silico analysis of cancer using Survivin as drug target
Author: Gobind Ram, Ramandeep Kaur

Survivin is a member of inhibitor of apoptosis protein family and plays an important role in cell division. This protein is undetectable in normal cells but is highly expressed in variety of human cancers including that of colon, breast, lung, pancreas and stomach that makes it a potential target for cancer treatment. Survivin, 142 amino acid proteins, is encoded by gene located on human chromosome 17 at band q25. It contains a baculovirus inhibitor of apoptosis repeat (BIR) protein domain that consists of 4 α-helices and 3-beta sheets. This BIR domain is essential for anti- apoptotic activity. Apoptosis is programmed cell death which maintains equivalence between the cell division and cell death. Survivin mainly inhibit caspases (cystein dependent aspartate directed proteases) that are involved in apoptosis and thus suppresses the apoptosis. It also acts as a subunit of chromosomal passenger complex that is required for proper chromosomal segregation and cytokinesis. Thus, Survivin plays dual role in spindle monitoring at mitosis and ability to inhibit apoptosis through the inhibition of caspases. In silico analysis was performed for homology search, motif and domain prediction and validation of model to the level of drug designing. Here we choose a ligand from zinc database and its drug properties like drug likeliness, solubility, clogP, drug score etc. were checked by Osiris property explorer and via Mol Inspiration. Docking was performed by using Hex software and Autodock Vina tool.

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