Paper Details

Name: Inderjeet Kaur, Vasvi Prabhakar
Email: kaurinder@lkc.ac.in
Country: India

Year: 2016
Volume: Volume-3, Issue-1

The antimicrobial activities of four disinfectants Sodium Chlorite, Iodine, Tetra butyl ammonium hydrogen and Crystal violet against Escherichia coli and Bacillus subtilis were investigated. These microorganisms were selected to test the Bactericidal activity of Disinfectants as these are frequent surface contaminants among the healthcare settings. Their efficacies were determined using Agar well diffusion method and Broth dilution method (for determination of minimum inhibitory concentration- MIC) at different concentrations of the test disinfectants. The results were recorded as diameter of zone of inhibition (mm) in agar well diffusion method and plate counting was performed in Broth dilution method. Different pathogens responded differently to different concentrations of disinfectants. The test disinfectants used in this study has been confirmed to be very effective, but their rate of efficiency varies due to the differences in their chemical composition and mechanism of action. This study revealed that Crystal Violet and Tetra butyl ammonium hydrogen sulfate have an excellent Bactericidal activity against both Gram positive (Bacillus subtilis) and gram negative (Escherichia coli) bacteria as compared to the others. The use of
Tetra butyl ammonium hydrogen sulfate at 6% and Crystal Violet at 1% against B. subtilis showed strong bactericidal efficacy with a zone of inhibition of 35 mm and 37 mm, respectively as compared to the others. The outcome of this study confirmed that Sodium Chlorite was relatively more effective against both the test organisms than Iodine. The maximum diameter of zone of inhibition observed for Sodium Chlorite against E. coli 40 mm. The least inhibition was seen with Iodine against both bacterial strains. Thus the efficacy of disinfectants in descending order is Crystal Violet ˃Tetra butyl ammonium hydrogen sulfate ˃ Sodium Chlorite ˃ and Iodine for both cultures. The result of Broth dilution method also show that Crystal violet, Tetra butyl ammonium hydrogen sulfate and sodium chlorite are most effective disinfectants on tested microorganisms, while iodine showed least bactericidal activity even at higher concentrations against both organisms. Thus, to ensure disinfectants efficacy, tests should be carried out on new disinfectant products and also further studies should be carried out about disinfectants.